Paper Chromatography Experiment Report | Examples and Samples

 

thin layer chromatography lab report discussion

the TLC plate. In this experiment, thin layer chromatography is used to identify compounds. In order to allow for good separation of all three compounds for correct identification, a solvent-pair was used. The solvent was chosen to be 95% hexanes and 5% ethanol. The hexanes allows for separation of the polar and non-polar compounds and the ethanol allows for the polar compounds to rise up the %(28). Thin layer chromatography (TLC) is a chromatography technique used to separate mixtures.[1] Thin layer chromatography is performed on a sheet of glass, plastic, or aluminum foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose (blotter paper). Mar 26,  · Thin Layer & Column Chromatography By: Lisa Mickey. Introduction Thin layer chromatography (also known as TLC) is the physical separation of a mixture into its individual components by distributing the components between a stationary phase (the porous TLC plate) and a mobile phase (the solvent that moves through the stationary phase and carries the material that needs .


(PDF) THIN LAYER CHROMATOGRAPHY | Mark Buluma Eugine - basqupiats.gq


Book: Accounting. There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. Procedure Thin layer chromatography lab report discussion method is a method of separating mixtures of two or more compounds. Two phases are important in this method; one that is stationary and one that is moving. Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two different phases between which are to be partitioned.

The mixture is observed when thin layer chromatography lab report discussion is in two different phase; a solid stationary phase and a liquid moving phase.

A thin layer chromatograph plate is used to identify drug component under UV. Results Sample Distance between start line and spot Distance between start line and front line Rf Aspirin 3. For example, if a compound contains carboxylic acid, alcohol or amine functional groups, it should be very polar, thin layer chromatography lab report discussion. If a compound contains ketone, aldehyde, ester, amide, or ether functional groups, it should be polar.

Lastly, if a compound contains methyl or alkyl substituent functional groups, it should be non-polar. Thus, since caffeine has an amine and amide group, its relative polarity is the highest in comparison to the other three ingredients. By understanding the relative polarities of the analgesic drugs, one could determine how the compounds will behave during the TLC separation. The stationary phase, silica gel in this experiment, is more polar, causing more polar components of a mixture to be more attracted to it, thin layer chromatography lab report discussion.

Thus, the more polar components will move more slowly and the less polar components will move faster on the TLC plates, having the highest Rf values. That is why in this particular experiment, caffeine, the most polar compound, has the smallest Rf value of 0.

Conclusion In conclusion thin layer chromatography is a useful technique when trying to identify compounds and see how they separate. It is also a useful tool to see how polar or nonpolar a compound is. Overall the lab was very successful and the results that were found were solid. Downloading text is forbidden on this website.

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Thin Layer Chromatography Lab Report - words | Study Guides and Book Summaries

 

thin layer chromatography lab report discussion

 

Thin Layer Chromatography Introduction Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is that there is . the TLC plate. In this experiment, thin layer chromatography is used to identify compounds. In order to allow for good separation of all three compounds for correct identification, a solvent-pair was used. The solvent was chosen to be 95% hexanes and 5% ethanol. The hexanes allows for separation of the polar and non-polar compounds and the ethanol allows for the polar compounds to rise up the %(28). Experiment 3 - TLC - Discussion - Free download as Word Doc .doc /.docx), PDF File .pdf), Text File .txt) or read online for free. paper chromatography lab report. aspirin and ibuprofen by using the thin layer chromatography (TLC). In this case, there are four chemical compounds have to be separated, thereby, choosing a suitable /5(9).